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Water quality expert inspecting plumbing and dead legs in sterile processing after failing bacterial water tests

When Bacterial Water Tests Failed — and Water Wasn’t the Only Suspect

The renovation was complete. The washers were installed. The sinks gleamed. The piping was new. Everything looked ready. But the water tests kept failing. Not every month. Just often enough to create doubt. One point of use would pass. Another would fail. The next month it would reverse. The department delayed moving into the newly renovated space. Weeks became months. Months became two years. That’s when Len Sparks was called in.


The First Question

When bacterial testing fails in sterile processing, the instinct is immediate: “The water system is contaminated.” And sometimes, that’s true.  But as Len often says:

“These are really challenging situations. You have to step back and look at best practices first.”

Before blaming anything, he asks three foundational questions:

  1. Are you disinfecting your critical water on a regular schedule?

  2. Are you flushing points of use routinely?

  3. What temperature is your water during idle periods?

Because water, like milk, doesn’t improve when it sits.

“Just like milk sitting on the counter will spoil faster at 80 degrees than 60 degrees, water behaves the same way.”


Clue #1: The Dead Leg

The facility proudly described their recirculating critical water loop. But when Len examined the sinks, he noticed something. Three-bay sinks connected to the loop — but between the wall and the faucet, three to four feet of tubing. Then a solenoid-controlled faucet underneath the sink. Another few feet of tubing. Water only moved when someone pressed a button. No programmable flushing. No automatic circulation to the faucet head.

In plumbing design, the rule of thumb for a dead leg is three to five times the pipe diameter — often just a few inches. This wasn’t inches. This was feet.

“Often people think a dead leg is piping behind the wall. But that coiled hose at your spray gun – that acts like a dead leg too.”

Stagnant water is biofilm’s favorite environment. And once biofilm attaches, surface disinfection removes only the outer layers. The inner layers remain anchored. Growth accelerates. That explained the pattern: passing one month, failing the next — with failures appearing more quickly over time.


Clue #2: No Preventative Disinfection

The system had UV. It had a final filter. It recirculated. The assumption: “We don’t need to disinfect.”

But ST108 guidance and best practice recommendations are clear: routine disinfection is preventative, not reactive. This department had gone 12 months without disinfecting the system. Biofilm doesn’t wait for a failure report. It establishes quietly.


Clue #3: The Testing Process

Then Len looked at how water samples were being collected. The company the hospital hired used no gloves. Hands contacting ports and faucets. Samples shipped overnight in summer heat — without proper chilling.

“It’s like drawing milk and putting it in FedEx in August. By the next morning, is that milk still good?”

The water leaving the facility wasn’t necessarily the water being measured at the lab. Temperatures reached nearly 90°F during shipping. Bacteria multiplied in transit. False elevation. False data. False conclusions. They weren’t testing the system. They were testing handling errors.


The Real Culprit

It wasn’t one failure. It was a conspiracy of small oversights:

  • Dead legs at sinks
  • Idle renovation period with no flushing
  • No routine disinfection
  • Improper sample handling
  • Warm shipping conditions

Each issue alone might not cause a crisis. Together, they stalled a department for two years.


The Bigger Lesson

When bacterial water tests fail, escalation is natural. But investigation must be disciplined. Ask:

  • Has this system been sitting unused?

  • Are all points of use flushed daily?

  • Are spray guns creating stagnation?

  • Is disinfection preventative or reactive?

  • Are sampling protocols audit-ready?

Because sometimes the system isn’t contaminated. It’s stagnant, mishandled, or misunderstood.


The Detective Mindset

Water quality problems rarely announce themselves clearly. They hide in:

  • Tubing lengths

  • Faucet design

  • Idle equipment

  • Maintenance assumptions

  • Sampling technique

And like any good detective, Len doesn’t chase noise. He follows process. As he often reminds departments:

“Don’t let it sit.”

Water that moves is easier to manage than water that rests.


Closing the Case

Once flushing protocols were established, dead legs were addressed, disinfection became routine, and sampling procedures were corrected, the failures stopped. The department moved in. The mystery dissolved.


Final Question

If your next bacterial test fails, will you only blame the water or will you investigate the system around it? In sterile processing, water quality isn’t just about purity. It’s about design, maintenance, and methodology. And the clues are usually closer than you think.

If you need detective-level help solving SPD water quality issues, Contact Us

Len was invited to be on the ANSI/AAMI committee that drafted ST108
Len Sparks, COO